The common features of the response to NGC treatment were that hepatocytes appeared to be the only cell population affected and the induction of oxidative stress where observed, with one exception thioacetamide, TAA , occurred in the absence of toxicity data not shown. Oxidative stress in the development of liver cirrhosis: Therefore in contrast to the current models, these data suggest that oxidative stress is not a key determinant in the mechanism of nongenotoxic carcinogenesis but may contribute to the effects in a compound-specific manner. In this study, acute dosing with NAF induced HO-1 reporter and protein expression in HOD mouse liver suggesting direct induction of oxidative stress in hepatocytes , while WY had little effect on the reporter but did induce a slight increase in HO-1 protein. Receive exclusive offers and updates from Oxford Academic.
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Each NGC treatment yielded distinct patterns of LacZ reporter expression, both in terms of intensity and localization camerln the liver. To validate the utility of the model, we investigated whether compounds which induce oxidative stress activated the HO-1 reporter. Having demonstrated induction of the HO-1 reporter by CdCl 2 and EQ, we used a panel of 7 NGCs which induce tumors via different mechanisms to characterize their capacity to induce oxidative stress. There is little evidence that CPA, which is an androgen receptor antagonist and PXR ligand, can induce oxidative stress and this is confirmed by our study.
However, although there was a slight increase in the number of cells staining for LacZ Fig. Microsomal fractions were prepared from frozen liver tissue according to standard procedures.
A-H, Detection of LacZ in liver sections by histochemical staining. A toxicogenomic approach for the prediction of murine hepatocarcinogenesis using ensemble feature selection.
Close mobile search navigation Article navigation. Identification of Dlk1-Dio3 imprinted gene cluster noncoding RNAs as novel candidate biomarkers for liver tumor promotion.
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Genotoxic carcinogens are relatively easy to distinguish by means of tests for DNA damage and mutagenesis, but nongenotoxic carcinogens NGCs induce tumors by various alternative mechanisms and can be difficult to identify, other than through classic rodent bioassays.
The weak induction of Cyp3a would also be consistent with this Fig.
Vehicle ms received PBS. Dynamic changes in 5-hydroxymethylation signatures underpin early and late events in drug exposed liver. These have previously been shown to cross-react specifically with the murine counterparts of their target Ps. The sections were photographed under bright field conditions on a Zeiss Axiocam microscope; the resulting images were processed with AxioVision software Zeiss.
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Oxford University Press is a department of the University of Oxford. Related articles in PubMed Characteristics of nonalcoholic steatohepatitis among lean patients in Japan: A sample of the supernatant from the first centrifugation step lysate was retained. Time response and dose response of hepatic heme oxygenase-1 reporter expression in response to thioacetamide. Effect of a rhodium III complex. As expected, the liver: Both LacZ staining and expression of HO-1 protein were more evident in centrilobular regions of the liver after EQ treatment, and more generally distributed following administration of CdCl 2.
In this study, we examined the effects of a range of NGCs on oxidative stress through the application cameton an HO-1 reporter mouse, the measurement of endogenous HO-1 protein, and the activation of the Nrf2 signaling cascade. In addition to DCB, the only other compound to induce a marked level of HO-1 reporter expression at a dose equivalent to that used in long-term carcinogenicity studies was NAF Fig. This work has identified a number of cakeron CAR-mediated mechanisms.
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The resulting HO-1 reporter loci contain most of the HO-1 gene together with The data obtained demonstrate that although some nongenotoxic carcinogens can induce oxidative stress, it is not a dominant feature of the response to these compounds. However, our studies do not support a role for PBO-induced oxidative stress in its mechanism of carcinogenesis.
A-C, Detection of LacZ in liver camerln by histochemical staining. Thioacetamide-induced hepatocellular necrosis is attenuated in diet-induced obese mice. Administration of this compound resulted in strong zonal induction of the HO-1 promoter Fig.
A practical camegon to determine the amount of tissue to analyze using laser scanning cytometry. Very few LacZ-positive hepatocytes were observed 4 days after exposure, but a greater number was detected at 28 days.
Our conclusion from these experiments is that the effects of oxidative stress on NGC-induced tumorigenesis is likely to be compound specific and does not represent a universal feature of this process. Arrows indicate individual LacZ-positive cells.